![]() ![]() Our findings indicated despite a strong agreement shown between iPCR and ELISA methods, the diagnostic power of iPCR technique was more sensitive than ELISA test for detection of T. A value of 0.25 I.U./ml for serum IgG was the cut-off value to differentiating between positive and negative toxoplasmosis (with sensitivity 66.0% and specificity 60.0%). Kappa (κ) value coefficient, between the iPCR and ELISA (for IgG) showed a strong agreement (0.360, p value < 0.001). While, 59 (72.8%) of 81 samples were detected positive using the iPCR technique. Moreover, of 81 specimens, 42 (51.9%) and 39 (48.1%) samples had low-avidity IgG and high-avidity IgG by the IgG avidity kit, respectively. gondii in the ELISA method, respectively. Of 81 samples, 4 (4.9%) and 30 (37%) cases were be found positive for IgM and IgG against T. The set-up of iPCR was carried out and then, serum IgG of subjects were detected using the iPCR method. In addition, detection of acute and chronic toxoplasmosis was performed via the ELISA IgG avidity. gondii were measured by the ELISA method in 81 participants. Immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against T. Hence, the current investigation was designed to compare diagnostic power of immuno-polymerase chain reaction (iPCR) and enzyme-linked immunosorbent assay (ELISA) methods for detection of T. gondii persist for years, and can act as a reliable serological biomarker for the diagnosis of previous exposure to this parasite. Since the prevalence of this parasite is high in many products (meat and milk, marine products, and vegetables), it is necessary to standardize detection methods, as well as implement control measures. As a result, emerging techniques are being developed that are aimed at the detection of multiple parasites simultaneously that would make their application more efficient in the industry. The main detection methods are bioassays, cell culture, molecular and microscopic techniques, and serological methods, but some of these do not have applicability in the food industry. gondii in different food products, and the control measures. In this review, we analyze the current detection methods, the prevalence of T. gondii, currently there are no standardized methods for its detection in the food industry. Despite the great health problems that are caused by T. Recently, contamination has been detected in fresh products with oocysts and marine products. gondii tissue cysts, and dairy products with tachyzoites. The consumption of contaminated food is the main source of infection. Toxoplasma gondii is an obligate intracellular parasite that causes toxoplasmosis, with approximately one third of the population around the world seropositive. ![]()
0 Comments
Leave a Reply. |
Details
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |